Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
3.3K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
Experimental RNAi02:15

Experimental RNAi

7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K
Types of RNA01:20

Types of RNA

8.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct roles of AdipoR1 and AdipoR2 in modulating neuronal firing homeostasis in response to adiponectin and AdipoRon.

Cell communication and signaling : CCS·2026
Same author

Molecular and computational analysis of conserved outer membranes (oprI and oprL) in MDR clinical isolates of Pseudomonas aeruginosa as potential vaccine targets from Lahore, Pakistan.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Isolation and characterization of <i>Novosphingobium oxfordense</i> sp. nov. and <i>Novosphingobium mississippiense</i> sp. nov. from soil, with LC-MS/MS and genome-based investigation of their glycosphingolipid production.

Frontiers in microbiology·2026
Same author

Investigating the versatility of cytochalasan cytochrome P450 monooxygenases using combinatorial biosynthesis reveals stereochemical restrictions.

Fungal biology and biotechnology·2026
Same author

Autophagy activation by urolithin-a derivative UA-36 mitigates Friedreich's ataxia pathologies induced by frataxin deficiency.

Molecular biomedicine·2026
Same author

JNK inhibition suppresses microglial NLRP3 activation and oxidative stress but unexpectedly worsens pain in diabetic neuropathy: insights from a combined in vitro and in vivo pharmacological study.

Neurochemistry international·2026

Related Experiment Video

Updated: Dec 24, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.1K

Prospective Advances in Non-coding RNAs Investigation.

Muhammad Ishtiaq Jan1, Tahir Ali1, Ayesha Ishtiaq1

  • 1Department of Biochemistry, Signal Transduction Laboratory, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Advances in Experimental Medicine and Biology
|April 15, 2020
PubMed
Summary

Non-coding RNAs (ncRNAs) are crucial in cellular processes and diseases like heart conditions. Further research into ncRNA functions and therapeutics can lead to advanced diagnostics and treatments for cardiovascular diseases (CVDs).

Keywords:
CVD managementDiagnosticsTherapeuticsmiRNAncRNA

More Related Videos

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

24.2K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

978

Related Experiment Videos

Last Updated: Dec 24, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.1K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

24.2K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

978

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Non-coding RNAs (ncRNAs) are integral to cellular functions and disease development.
  • ncRNAs are implicated in various pathologies, including cardiovascular diseases (CVDs).

Purpose of the Study:

  • To explore the relationship between ncRNA functions and their role in cardiovascular disease management.
  • To highlight prospective advances in non-coding RNA investigation, particularly microRNAs (miRNAs), for CVDs.

Main Methods:

  • Literature review and synthesis of current research on ncRNAs and cardiovascular conditions.
  • Analysis of the molecular basis and functional mechanisms of ncRNAs in disease.

Main Results:

  • ncRNAs significantly influence physiological processes and pathological alterations in CVDs.
  • MicroRNAs (miRNAs) show promise as therapeutic targets and diagnostic markers for cardiovascular conditions.

Conclusions:

  • The field of ncRNA therapeutics offers exciting opportunities for developing novel treatments and diagnostics for CVDs.
  • In-depth understanding of ncRNA molecular biology, mechanisms, and chemical formulations is essential for advancing CVD management.