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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Video

Updated: Dec 24, 2025

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems.

Ryuji Igarashi1,2,3, Takuma Sugi4, Shingo Sotoma5,6

  • 1Institute for Quantum Life Science, National Institute for Quantum and Radiological Science and Technology, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan.

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|April 15, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a new technique to track nanoscale three-degrees-of-freedom (3-DoF) rotation in biomolecules and live cells. This method visualizes 3-D rotational dynamics, offering new insights into biological mechanisms at the molecular and cellular levels.

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Three-degrees-of-freedom (3-DoF) rotation, encompassing roll, pitch, and yaw, is crucial for various applications but challenging to measure at the nanoscale.
  • Existing methods lack the sensitivity to track 3-DoF rotation in biomolecules and live cells.

Purpose of the Study:

  • To develop and demonstrate a novel technique for visualizing and measuring nanoscale 3-DoF rotation in biological systems.
  • To apply this method to understand the dynamics of motor proteins, membrane proteins, and organisms.

Main Methods:

  • Utilized nitrogen-vacancy (NV) centers in fluorescent nanodiamonds for sensing.
  • Employed a tomographic vector magnetometry technique to track 3-D rotational motion.
  • Attached nanodiamonds to specific biomolecules (F1-ATPase, membrane proteins) and observed cellular/organismal movements (C. elegans).

Main Results:

  • Successfully visualized the 3-step rotation of F1-ATPase, revealing delays in ATP binding/ADP release.
  • Correlated 3D rotational motion of membrane proteins in live cells with intracellular cytoskeletal density.
  • Tracked nonrandom motions within the intestine of *Caenorhabditis elegans*.

Conclusions:

  • The nanodiamond-based tomographic vector magnetometry method enables nanoscale 3-DoF rotation tracking in vitro, in cells, and in vivo.
  • This technique provides a new perspective on microscopic biological samples, enhancing understanding of functional mechanisms driven by nanoscale dynamics.