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LARP1 isoform expression in human cancer cell lines
Hagen Schwenzer1, Mai Abdel Mouti1, Pia Neubert1
1Department of Oncology, University of Oxford, Oxford, UK.
RNA Biology
|April 15, 2020
Summary
The long LI-LARP1 isoform is the main form of the LARP1 protein, while the short SI-LARP1 isoform is epigenetically repressed. This finding clarifies LARP1 expression in cancer cells.
Area of Science:
- Molecular Biology
- Cancer Biology
- Epigenetics
Background:
- LARP1 is an oncogenic protein crucial for ribosome biogenesis and cancer cell survival.
- Discrepancies exist regarding the canonical LARP1 protein isoform in scientific literature.
Purpose of the Study:
- To determine the predominantly expressed LARP1 protein isoform.
- To investigate the expression regulation of different LARP1 isoforms.
Main Methods:
- Biochemical assays
- Cellular assays
- Analysis of gene expression and epigenetic regulation
Main Results:
- The long LI-LARP1 isoform (NM_033551.3) is the dominantly expressed form.
- The short SI-LARP1 isoform (NM_015315.5) is epigenetically repressed, an evolutionarily conserved mechanism.
- Multiple nuclear-retained LARP1 mRNA isoforms suggest context-specific expression regulation.
Conclusions:
- LI-LARP1 is the canonical LARP1 isoform.
- Epigenetic repression of SI-LARP1 is a conserved mechanism.
- Cellular control over LARP1 mRNA splicing and expression indicates context-dependent regulation.
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