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Portal flow and mitochondrial function during extrahepatic cholestasis.

O Castro-e-Silva Júnior1, R Ceneviva, J E Roselino

  • 1Departamento de Cirurgia e Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|January 1, 1988
PubMed
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Extrahepatic cholestasis, caused by bile duct ligation, significantly reduces portal blood flow and liver mitochondrial function in rats. This suggests cholestasis may lead to liver ischemia due to an energy supply-demand imbalance.

Area of Science:

  • Hepatology
  • Gastroenterology
  • Physiology

Background:

  • Extrahepatic obstruction leads to cholestasis, impacting liver function.
  • Understanding the effects of cholestasis on portal hemodynamics and cellular energy metabolism is crucial.

Purpose of the Study:

  • To investigate the impact of extrahepatic obstruction on portal blood flow.
  • To assess mitochondrial and hepatic function in the context of cholestasis.
  • To determine if cholestasis induces liver ischemia.

Main Methods:

  • Bile duct ligation was performed in male Wistar rats.
  • Portal blood flow was measured 7 days post-ligation.
  • Mitochondrial respiratory control ratio and serum alanine-aminotransferase levels were assessed.

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  • Results were compared to sham-operated controls.

    • Main Results:

    • Portal blood flow decreased by 50% in the cholestatic group (P < 0.01).
    • Mitochondrial respiratory control ratio was reduced by 35% (P < 0.01).
    • Significantly elevated serum alanine-aminotransferase levels were observed in cholestatic rats (P < 0.01).

    Conclusions:

    • Extrahepatic cholestasis impairs portal blood flow and hepatic mitochondrial function.
    • These findings suggest cholestasis may induce liver ischemia.
    • An imbalance between energy supply and demand in the liver likely contributes to cholestasis-induced injury.