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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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A Scoring Method for Immunohistochemical Staining on Ki67.

Rui Guo1, Li Ma1, Xiomei Bai1

  • 1Department of Pathology.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|April 15, 2020
PubMed
Summary

A new scoring method enhances the interpretation of immunohistochemistry (IHC) staining for nuclear-type molecules. This reliable grading system improves diagnostic accuracy and supports personalized therapy decisions.

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Area of Science:

  • Pathology
  • Biomedical Imaging
  • Cancer Diagnostics

Background:

  • Accurate interpretation of immunohistochemistry (IHC) staining is vital for disease diagnosis.
  • Existing IHC scoring methods can lack standardization and reproducibility.
  • Nuclear-type molecule staining requires precise quantification for clinical utility.

Purpose of the Study:

  • To introduce a novel, standardized scoring method for IHC staining of nuclear-type molecules.
  • To establish a reliable and reproducible grading system for IHC results.
  • To improve the clinical evaluation of IHC data for personalized medicine.

Main Methods:

  • Subdividing samples into histological subtypes (covered, mosaic, mesenchymal).
  • Implementing an 11-grade scoring system (0 to X) using a cut-off of moderate-positive (2+) cells and 10% differential expression.
  • Re-evaluating Ki67 staining in 88 carcinoma specimens to assess ease, reliability, and reproducibility among pathologists.

Main Results:

  • The novel IHC scoring method demonstrated high consistency ratios across subtypes, especially when using a 10% cut-off threshold (92.5%-96.8%).
  • Excellent agreement was observed among 5 independent pathologists in correlating counts.
  • The method proved easy, reliable, and reproducible in re-evaluating Ki67 staining.

Conclusions:

  • The proposed IHC scoring method offers a novel, simple, reliable, and reproducible grading system.
  • This practical approach enhances the accurate interpretation of IHC staining data.
  • The method has the potential to significantly improve clinical evaluation and guide personalized therapy.