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Sequential variation in genomic RNA patterns of human rotaviruses isolated from infantile gastroenteritis

F J Buesa1, M Duato1, C Gimeno1

  • 1Departamento de Microbiología, Hospital Clínico Universitario, Facultad de Mediana, Universidad de Valencia, 46010 Valencia (Spain).

Annales De L'Institut Pasteur. Virology
|April 15, 2020
PubMed

Insights

This study tracked rotavirus RNA patterns in young children with gastroenteritis. A dominant pattern emerged early in the season, followed by diverse patterns later, with all strains belonging to subgroup II.

Area of Science:

  • Virology
  • Molecular Biology
  • Pediatric Infectious Diseases

Background:

  • Rotavirus is a leading cause of acute gastroenteritis in infants and young children globally.
  • Understanding the genetic diversity of rotavirus strains is crucial for developing effective vaccines and control strategies.
  • Previous studies have highlighted seasonal variations in rotavirus epidemiology, but detailed RNA analysis provides deeper insights.

Purpose of the Study:

  • To investigate the incidence and RNA electrophoretic patterns of rotavirus isolates from children with acute gastroenteritis.
  • To analyze the temporal dynamics of rotavirus RNA profiles during a specific outbreak season.
  • To subgroup the identified rotavirus isolates using immunoassay techniques.

Main Methods:

  • Collection of rotavirus isolates from pediatric patients with acute gastroenteritis between October 1985 and April 1986.
  • Polyacrylamide gel electrophoresis (PAGE) followed by silver staining for viral RNA analysis.
  • Double-sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies for rotavirus subgrouping.

Main Results:

  • Fourteen distinct rotavirus RNA electrophoretypes were identified.
  • A single dominant electrophoretype predominated during the early months of the rotavirus season.
  • A wider array of RNA patterns emerged later in the study period, with all isolates confirmed as subgroup II. Mixed electrophoretypes were observed in 8.16% of cases.

Conclusions:

  • Rotavirus outbreaks can exhibit significant temporal variations in RNA electrophoretic profiles.
  • The predominance of a single electrophoretype early in the season suggests a potential common source or rapid spread of a specific strain.
  • All identified strains belonging to subgroup II indicate a potential commonality in circulating rotavirus strains during the study period.
Keywords:
Molecular epidemiology, Genomic RNA patterns, Sequential variation, Electrophoretypes, ChildrenRotavirus, RNA, Gastroenteritis

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