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Real Time RT-PCR

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Related Experiment Video

Updated: Dec 24, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Compact optical diagnostic device for isothermal nucleic acids amplification.

Szu-Yuan Lee^1,2,3, Jhen-Gang Huang¹, Tsung-Liang Chuang¹

¹Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan, ROC.

Sensors and Actuators. B, Chemical

|April 15, 2020

Summary

This study introduces an integrated device for rapid hepatitis B virus (HBV) DNA detection using loop-mediated isothermal amplification (LAMP). The device successfully identifies HBV DNA in clinical samples within one hour.

Keywords:

Diagnostic device Hepatitis B virus Isothermal LAMP

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Last Updated: Dec 24, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

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Published on: February 3, 2021

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Published on: November 15, 2017

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

Area of Science:

Biotechnology
Molecular Diagnostics
Medical Devices

Background:

Hepatitis B virus (HBV) DNA amplification is crucial for diagnosis.
Loop-mediated isothermal amplification (LAMP) offers a rapid method for DNA amplification.
Existing methods require complex laboratory setups, limiting point-of-care applications.

Purpose of the Study:

To develop an integrated device for simultaneous amplification and detection of HBV DNA.
To enable rapid, real-time monitoring of HBV DNA levels.
To create a user-friendly platform for point-of-care HBV diagnostics.

Main Methods:

Development of a disposable polymethyl methacrylate (PMMA) micro-reactor.
Integration with a temperature-regulated optical detection unit for turbidity monitoring.
Utilizing magnesium pyrophosphate precipitation as a detection signal for LAMP by-products.

Main Results:

Established a correlation curve (R² = 0.99) between turbidity and pyrophosphate concentration.
Developed a standard curve (R² = 0.96) for HBV DNA quantification using LAMP.
Successfully detected HBV DNA in seven clinical serum specimens within one hour.

Conclusions:

The integrated device provides a sensitive and rapid method for HBV DNA detection.
The device demonstrates potential for effective point-of-care diagnostics.
This technology can significantly improve the speed and accessibility of HBV testing.