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Related Experiment Video

Updated: Dec 24, 2025

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Yeast surface display-based microfluidic immunoassay.

Jing Wang¹, Danhui Cheng¹, Jay Kwok-Lun Chan²

¹Bioengineering Graduate Program, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.

Sensors and Actuators. B, Chemical

|April 15, 2020

Summary

This summary is machine-generated.

This study introduces a novel microfluidic immunoassay using yeast surface display (YSD) for sensitive antibody detection. This platform offers a portable and cost-effective alternative to traditional methods, enabling future point-of-care diagnostics.

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

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Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Traditional immunoassays often require complex and expensive equipment like flow cytometers.
There is a need for sensitive, portable, and cost-effective diagnostic platforms.

Purpose of the Study:

To develop a novel microfluidic immunoassay platform.
To combine yeast surface display (YSD) with microfluidics for enhanced antibody detection.
To create a portable and sensitive diagnostic tool.

Main Methods:

Engineered yeast cells displaying specific antigens were used as fluorescent labels.
Yeast surface display (YSD) was integrated with a microfluidic chip.
A functionalized micro pillar array (MPA) was incorporated to improve sensitivity and eliminate the need for pumping.

Main Results:

The platform successfully detected target antibodies with a detection limit of 5 ng/mL.
Multiplex immunoassay was achieved by using yeast cells with different fluorescent proteins.
The microfluidic setup eliminated the need for a flow cytometer.

Conclusions:

The developed YSD-based microfluidic immunoassay is sensitive and efficient.
This platform offers a promising alternative for portable diagnostics and clinical applications.
Future integration into microfluidic systems can enable point-of-care testing.