Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD98hc-targeted antibody shuttles for central nervous system delivery with broad cross-species reactivity.

Nature biomedical engineering·2026

Same author

Aerosol flow-driven instability and droplet-interfacial stabilization of mRNA lipid nanoparticles during mesh nebulization.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

A Single CeO<sub>2</sub>/Polydopamine/Multiwalled Carbon Nanotube Hybrid Sensing Platform for Concurrent Voltammetric Monitoring of Benzo[a]pyrene, Pyrene, and Fluorene in Particulate Matter.

ACS sensors·2026

Same author

Recent Advances in Nanoscale Sprayable Hydrogels for Cancer Management: Nanoparticle-Loaded Formulations for Therapy and Prevention.

International journal of nanomedicine·2026

Same author

Chromium nitride nanozyme-enhanced lateral flow immunoassay for sensitive and selective detection of transglutaminase 2: Proof-of-concept toward liver cancer-related biomarker evaluation.

Biosensors & bioelectronics·2026

Same author

Effects of Oral Health and Functional Characteristics on Taste Sensitivity in Older Adults: Comparative Analysis Using Solution and Taste Strip Tests.

Journal of oral rehabilitation·2026

Search research articles

Related Experiment Video

Updated: Dec 24, 2025

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Published on: September 17, 2021

Bioaffinity detection of pathogens on surfaces.

Alastair W Wark¹, Jaeyoung Lee², Suhee Kim³

¹Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK.

Journal of Industrial and Engineering Chemistry (Seoul, Korea)

|April 15, 2020

Summary

This summary is machine-generated.

This study reviews surface bioaffinity detection for rapid pathogen identification. It covers probe molecules, current biosensors, and emerging technologies for sensitive and selective pathogen sensing.

Keywords:

Lab-on-a-chip Label-free detection Nanomaterials Pathogen detection Surface biosensors

More Related Videos

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Related Experiment Videos

Last Updated: Dec 24, 2025

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Published on: September 17, 2021

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Published on: September 17, 2017

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Area of Science:

Analytical Chemistry
Biotechnology
Nanotechnology

Background:

Rapid and sensitive pathogen detection is crucial in complex environments.
Surface-based platforms offer simultaneous monitoring of multiple bioaffinity interactions.
Advancements in signal transduction and amplification are key for improved detection.

Purpose of the Study:

To review the latest advances in surface bioaffinity detection of pathogens.
To discuss probe molecules, immobilization chemistries, and current biosensor examples.
To explore emerging technologies for next-generation pathogen sensors.

Main Methods:

Overview of probe molecules (antibodies, proteins, aptamers) for pathogen specificity.
Discussion of surface attachment chemistries for probe immobilization on substrates.
Analysis of current and emerging surface biosensor technologies.

Main Results:

Selection of appropriate probe molecules and immobilization strategies is critical.
Current surface biosensors demonstrate significant progress in pathogen detection.
Emerging technologies show high promise for future sensitive and selective pathogen sensing.

Conclusions:

Surface bioaffinity detection is a powerful approach for pathogen sensing.
Continued innovation in probe design, surface chemistry, and signal transduction is essential.
Future research will likely focus on integrated and highly sensitive pathogen detection systems.