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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Foodborne viruses.

Albert Bosch1,2, Rosa M Pintó1,2, Susana Guix1,2

  • 1Enteric Virus Group, Department of Microbiology, University of Barcelona, Avda Diagonal 643, 08028 Barcelona, Spain.

Current Opinion in Food Science
|April 15, 2020
PubMed
Summary
This summary is machine-generated.

Foodborne viruses like norovirus and hepatitis A cause many outbreaks. New molecular detection methods are being developed for faster foodborne illness investigations and require validation.

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Area of Science:

  • Food safety and public health
  • Virology
  • Molecular diagnostics

Background:

  • Noroviruses and hepatitis A virus are leading causes of foodborne viral outbreaks.
  • Hepatitis E virus is an emerging zoonotic concern in food contamination.
  • Current molecular methods exist for detecting certain foodborne viruses, but viability assessment is pending.

Purpose of the Study:

  • To address the need for improved detection and characterization of foodborne viruses.
  • To highlight the importance of validating new rapid methods against established standards like ISO.
  • To emphasize the necessity of developing and harmonizing next-generation sequencing protocols for outbreak investigations.

Main Methods:

  • Review of existing molecular detection techniques for foodborne viruses.
  • Discussion of the limitations of current genome copy detection without viability assays.
  • Highlighting the ongoing development and validation requirements for novel diagnostic and sequencing methods.

Main Results:

  • Established molecular methods are available for norovirus and hepatitis A virus detection.
  • Viability assays are crucial for determining the health risks associated with detected viral loads.
  • New rapid methods and next-generation sequencing protocols are under development and require validation.

Conclusions:

  • Accurate detection and characterization of foodborne viruses are essential for public health.
  • Validation of new molecular methods against standards is necessary for reliable outbreak investigation.
  • Standardized protocols for advanced sequencing techniques will enhance the response to foodborne viral outbreaks.