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Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
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Estimation of Microbial Viability Using Flow Cytometry.

Hazel Davey1, Stéphane Guyot2

  • 1Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United Kingdom.

Current Protocols in Cytometry
|April 15, 2020
PubMed
Summary
This summary is machine-generated.

Measuring microbial viability is challenging. This study explores flow cytometry and viability stains as rapid alternatives to traditional culturability methods for assessing microbial health.

Keywords:
flow cytometrymicrobial viabilitymicrobiologyviability

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Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Microbial viability is difficult to define and measure.
  • Traditional methods like culturability are slow and time-consuming.
  • Rapid and quantitative assessment of microbial viability is needed.

Purpose of the Study:

  • To evaluate viability stains using flow cytometry for microbial analysis.
  • To develop protocols for routine microbial viability assessments.
  • To provide a basis for validating flow cytometry assays for specific microorganisms.

Main Methods:

  • Utilizing flow cytometry for rapid, quantitative measurements of dye uptake in microbial cells.
  • Exploiting viability stains to assess microbial cell status.
  • Developing and presenting protocols for flow cytometry-based viability assays.

Main Results:

  • Flow cytometry offers a rapid alternative to traditional culturability methods.
  • Viability stains can be effectively used with flow cytometry for microbial assessment.
  • Protocols are provided for implementing these assays on standard flow cytometer instruments.

Conclusions:

  • Flow cytometry and viability stains provide a faster approach to microbial viability assessment.
  • Careful validation of each assay with the specific microorganism is crucial.
  • These methods can support research, clinical, and service applications in microbiology.