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A natural deep eutectic solvent (NADES) as potential excipient in collagen-based products
Krister Gjestvang Grønlien1, Mona Elisabeth Pedersen2, Hanne Hjorth Tønnesen1
1Section for Pharmaceutics and Social Pharmacy, Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, NO-0316 Oslo, Norway.
International Journal of Biological Macromolecules
|April 15, 2020
Summary
Natural deep eutectic solvents (NADES) can dissolve collagen, an important protein for wound healing. This research explores collagen
Area of Science:
- Biomaterials Science
- Chemical Engineering
- Biochemistry
Background:
- Natural deep eutectic solvents (NADES) exhibit antibacterial properties, with potential applications in wound healing.
- Collagen, a key structural protein, possesses unique wound healing capabilities.
- Combining NADES and collagen may offer synergistic therapeutic benefits.
Purpose of the Study:
- To investigate the behavior and structural integrity of collagen within NADES solutions.
- To assess the potential of NADES as an excipient for collagen-based wound healing products.
Main Methods:
- Spectroscopic analysis
- Calorimetric analysis
- Viscosity measurements
- Evaluation of collagen (telo- and atelocollagen) in varying NADES concentrations and aqueous dilutions.
Main Results:
- Collagen exhibited variable unfolding depending on collagen type and NADES aqueous dilution.
- Both collagen types underwent unfolding in undiluted NADES.
- Collagen in highly diluted NADES showed structural stability, similar to dissolution in acetic acid.
Conclusions:
- NADES can dissolve collagen while potentially maintaining its structural properties, particularly in diluted forms.
- NADES demonstrate promise as a novel excipient for collagen-based biomaterials and wound healing applications.
- This study is the first to describe the solubility and structural changes of extracellular matrix proteins in NADES.
