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Characterizing the Microbiome of the Contracted Breast Capsule Using Next Generation Sequencing
Aesthetic Surgery Journal
|April 16, 2020
Summary
Next-generation sequencing (NGS) revealed bacterial DNA in over half of capsular contracture (CC) breast tissues. This study identifies key bacteria like E. coli and C. acnes, advancing understanding of CC development.
Area of Science:
- Microbiology
- Genomics
- Plastic Surgery
Background:
- Bacterial biofilms are implicated in capsular contracture (CC) after breast augmentation.
- Traditional culture methods offer limited insight into the breast tissue microbiome.
- Next-generation sequencing (NGS) provides comprehensive DNA analysis of microbial communities.
Purpose of the Study:
- To characterize the bacterial microbiome in breast implant capsules associated with CC using NGS.
- To identify specific bacterial and fungal species present in contracted breast capsules.
Main Methods:
- Evaluation of 32 patients with Baker grade III or IV CC following augmentation mammoplasty.
- Collection of tissue specimens from contracted capsules and intraoperative swabs.
- Analysis of samples using NGS technology at MicroGenDX Laboratories.
Main Results:
- Microbial DNA was detected in 56% of patients (22 of 53 samples).
- Sequencing identified 120 bacterial and 6 fungal species.
- Most frequent isolates included Escherichia coli, Diaphorobacter nitroreducens, Cutibacterium acnes, and Staphylococcus epidermidis.
Conclusions:
- NGS offers detailed characterization of the breast implant microbiome.
- Understanding this microbiome is crucial for elucidating the role of bacteria in CC development.
