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Updated: Dec 24, 2025

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Screening of copper and palladium-mediated reactions compatible with DNA-encoded chemical libraries
Nicholas Favalli1, Gabriele Bassi1, Tania Zanetti1
1Institute of Pharmaceutical Sciences, ETH Zürich, 8093 Zürich (Switzerland).
Abstract:
The construction of DNA-encoded chemical libraries (DECLs) crucially relies on the availability of chemical reactions, which are DNA-compatible and which exhibit high conversion rates for a large number of diverse substrates. In this work, we present our optimization and validation procedures for three copper and palladium-catalyzed reactions (Suzuki cross-coupling, Sonogashira cross-coupling and copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC)), which have been successfully used by our group for the construction of large encoded libraries.

