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miRNA-186 Improves Sepsis Induced Renal Injury Via PTEN/PI3K/AKT/P53 Pathway

Min Li1, Wei Li1, Feng-Qin Ren1

  • 1Department of Intensive Care Unit, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, 250013, China.

Open Medicine (Warsaw, Poland)
|April 16, 2020
PubMed
Abstract

Insights

MicroRNA-186 (miRNA-186) protects against sepsis-induced kidney injury by targeting the PTEN pathway. This study demonstrates miRNA-186

Area of Science:

  • Nephrology
  • Molecular Biology
  • Sepsis Research

Background:

  • Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection.
  • Renal injury is a common and severe complication of sepsis, leading to significant morbidity and mortality.
  • MicroRNAs (miRNAs) play crucial roles in regulating cellular processes and have emerged as potential therapeutic targets in various diseases, including sepsis-induced kidney injury.

Purpose of the Study:

  • To investigate the therapeutic effects of microRNA-186 (miRNA-186) on renal injury induced by sepsis.
  • To elucidate the underlying molecular mechanisms, specifically focusing on the interaction between miRNA-186 and Phosphatase and tensin homologous protein (PTEN).

Main Methods:

  • A sepsis model was established in Wistar rats using cecal ligation and puncture (CLP).
  • Rats were divided into Sham, Sepsis, and miRNA-186 treatment groups.
  • Kidney tissues were analyzed for histopathological changes and cell apoptosis using H&E and TUNEL assays, respectively.
  • Protein expression levels were quantified using Western blot (WB) analysis.

Main Results:

  • Sepsis induction led to significant renal histopathological damage and increased cell apoptosis compared to the Sham group (P<0.05).
  • Administration of miRNA-186 significantly ameliorated kidney damage and reduced cell apoptosis in septic rats compared to the Sepsis group (P<0.05).
  • Protein expression levels, including that of PTEN, were significantly altered among the groups, confirming PTEN as a target of miRNA-186.

Conclusions:

  • Overexpression of miRNA-186 demonstrates a protective effect against sepsis-induced renal injury.
  • The therapeutic benefits of miRNA-186 in sepsis-related kidney damage are mediated through the PTEN signaling pathway.
  • miRNA-186 represents a potential therapeutic strategy for managing sepsis-induced kidney injury.
Keywords:
apoptosismiRNA-186renal injury

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