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Updated: Dec 24, 2025

A Customizable Chamber for Measuring Cell Migration

A Customizable Chamber for Measuring Cell Migration

Published on: March 12, 2017

3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources.

Alexander P Haring¹, Emily G Thompson², Raymundo D Hernandez²

¹Department of Industrial and Systems Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.

Advanced Biosystems

|April 16, 2020

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A new 3D printed assay characterizes cell chemotaxis to multiple signals. Glioblastoma cells showed a stronger directional migration toward epidermal growth factor (EGF) than bradykinin (BK).

Area of Science:

Biomedical Engineering
Cell Biology
Cancer Research

Background:

Chemotaxis, or cell movement in response to chemical cues, is crucial for biological processes.
Glioblastoma cell migration is influenced by various chemoattractants, impacting tumor growth and metastasis.
Understanding glioblastoma cell navigation in complex chemical environments is vital for developing targeted therapies.

Purpose of the Study:

To develop and validate a 3D printed multiplexed competitive migration assay.
To characterize the chemotactic response of human glioblastoma cells to multiple, spatially opposing chemoattractants.
To investigate glioblastoma cell interactions with vasculature through chemotaxis.

Main Methods:

Fabrication of a 3D printed device enabling multiplexed competitive migration assays.

Keywords:

gradients microextrusion 3D printing microphysiological neural systems neural system-on-a-chip organ-on-a-chip

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Last Updated: Dec 24, 2025

A Customizable Chamber for Measuring Cell Migration

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Generation of spatially opposing chemotactic gradients using epidermal growth factor (EGF) and bradykinin (BK).

Quantification of glioblastoma cell migration direction and velocity in response to individual and competing chemoattractants.

Main Results:

The assay successfully characterized glioblastoma cell chemotaxis in the presence of multiple chemoattractants.
Human glioblastoma cells exhibited a preferential migration towards EGF (46% more cells) over BK when gradients opposed.
Cell migration velocities were reduced when exposed to competing chemoattractant gradients compared to single gradients.

Conclusions:

The 3D printed assay provides a versatile platform for studying complex chemotactic behaviors.
Glioblastoma cell migration is differentially regulated by EGF and BK, offering insights into tumor-vasculature interactions.
This technology can advance the understanding of cell migration in various biological contexts.