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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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Identification of microRNAs and their Endonucleolytic Cleavaged target mRNAs in colorectal cancer

Fangbin Zhou1,2, Donge Tang1, Yong Xu1

  • 1Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), 1017 North Rd Dongmen, Luohu District, Shenzhen, China.

BMC Cancer
|April 16, 2020
PubMed
Abstract

Insights

Colorectal cancer (CRC) research reveals microRNAs (miRNAs) cleave messenger RNAs (mRNAs). This study identified novel miRNA targets and potential prognostic markers like ezrin (EZR) for colorectal cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) is a leading global malignancy.
  • MicroRNAs (miRNAs) are implicated in CRC pathogenesis by regulating mRNA stability and translation.
  • The precise mechanism of miRNA-mediated mRNA cleavage in CRC requires further elucidation.

Purpose of the Study:

  • To investigate the mechanism of miRNA-directed mRNA cleavage in colorectal cancer.
  • To identify differentially expressed miRNAs (DEmiRNAs) and their target genes in CRC.
  • To explore potential prognostic biomarkers for CRC.

Main Methods:

  • High-throughput small RNA sequencing to profile miRNAs in colorectal cancer tissues (CTs) and adjacent normal tissues (CNs).
  • Degradome sequencing (DS) to identify cleaved target genes of miRNAs.
  • Bioinformatic analyses including DAVID for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Main Results:

  • Characterized 1278 known and 131 novel miRNAs, with 420 known and 8 novel DEmiRNAs identified between CTs and CNs.
  • Identified 9685 and 202 potential target transcripts for known and novel miRNAs, respectively, via DS.
  • Found 264 target genes for 85 DEmiRNAs involved in proteoglycans in cancer and AMP-activated protein kinase signaling pathways; ezrin (EZR) and HCLS1 showed prognostic potential.

Conclusions:

  • Endonucleolytic miRNA-directed mRNA cleavage is confirmed to occur in colorectal cancer.
  • Identified numerous potential target genes regulated by CRC-associated miRNAs.
  • Discovered potential prognostic markers, such as EZR and HCLS1, for colorectal cancer, enhancing understanding of miRNA-target interactions.
Keywords:
Colorectal CancerDegradome sequencingEndonucleolytic cleavageHigh-throughput small RNA sequencingmicroRNA

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