A Single Silicon Nanowire-Based Ratiometric Biosensor for Ca²⁺ at Various Locations in a Neuron

Ionic calcium (Ca²⁺) is an important second messenger in cells, particularly in the neuron. A deficiency or excess of Ca²⁺ would lead to neuronal apoptosis and further injury to the brain. For accurate analysis of intracellular Ca²⁺, a single silicon nanowire (SiNW)-based ratiometric biosensor was constructed by simultaneously anchoring Ru(bpy)₂(mcbpy-O-Su-ester)(PF₆)₂, as a reference molecule, and Fluo-3, as a response molecule, onto the surface of a single SiNW. The SiNW-based biosensor exhibits high sensitivity and favorable selectivity for detecting Ca²⁺. With the assistance of a micromanipulator and laser scanning confocal microscope, two single SiNW sensors were placed in the body and the neurites of an individual neuron to detect Ca²⁺. The difference between the concentrations of Ca²⁺ in the body and neurites was identified. The results from the present study provide new insights into Ca²⁺ in neurons at a high spatial resolution, and the strategy used in this study provides a new opportunity to investigate cellular metabolism by combining the advantages of a single-cell detection technique and physiology.