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Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode.

Myungsang Park1, Yesol Song1, Ki Jun Kim1

  • 1Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea.

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|April 17, 2020
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Summary

This study presents a new electrochemical sensor for detecting immunoglobulin E (IgE) in serum, crucial for allergy diagnosis. The sensor utilizes ferrocene-modified self-assembled monolayers for enhanced signal amplification, offering a sensitive method for allergy testing.

Keywords:
electrocatalytic reactionelectrochemical impedance spectroscopyferrocenehuman immunoglobulin Eindium tin oxide (ITO)

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Immunology

Background:

  • Serum immunoglobulin E (IgE) levels are critical biomarkers for diagnosing allergic diseases.
  • Existing methods for IgE detection can be complex and time-consuming.
  • Development of sensitive and rapid IgE detection platforms is essential for effective allergy management.

Purpose of the Study:

  • To develop and validate a novel electrochemical sensor for the quantitative detection of human IgE.
  • To utilize ferrocene-modified self-assembled monolayers for enhanced electrocatalytic signal amplification.
  • To assess the sensor's performance, including limit of detection and dynamic range.

Main Methods:

  • Fabrication of an indium tin oxide (ITO) electrode with ferrocene (Fc)-modified self-assembled monolayers (SAMs) as the sensing layer.
  • Immobilization of antibodies in a sandwich format: receptor antibody, target human IgE, and enzyme-conjugated secondary antibody.
  • Electrochemical analysis using redox cycling of the enzyme product (p-aminophenol) facilitated by Fc.
  • Electrochemical impedance spectroscopy (EIS) was also employed for IgE detection.

Main Results:

  • The Fc-modified SAMs facilitated efficient electron transfer and electrocatalytic signal amplification.
  • The electrochemical sensor demonstrated a limit of detection (LOD) of 3 IU/mL and a limit of quantification (LOQ) of 10 IU/mL.
  • A dynamic detection range of 10 IU/mL to 100 IU/mL was achieved for human IgE.

Conclusions:

  • The developed Fc-modified SAMs-based electrochemical sensor provides a sensitive and efficient platform for human IgE detection.
  • This biosensor holds promise for improved allergy diagnosis and monitoring.
  • The combination of SAMs and Fc-based electrocatalysis offers a viable strategy for developing advanced electrochemical biosensing systems.