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Updated: Dec 24, 2025
07:24
Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
Published on: July 9, 2021
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A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division
Lovorka Stojic1,2, Aaron T L Lun3,4, Patrice Mascalchi3,5
1Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge, CB2 0RE, UK. l.stojic@qmul.ac.uk.
Nature Communications
|April 17, 2020
Summary
Researchers discovered a long non-coding RNA (lncRNA), linc00899, that regulates cell division. Depleting this lncRNA delays mitosis by affecting microtubule dynamics through the TPPP/p25 protein.
Area of Science:
- Cell Biology
- Genomics
- Molecular Biology
Background:
- Genome stability depends on precise cell division processes, including mitosis and cytokinesis.
- Microtubules play a critical role in chromosome segregation during cell division.
Purpose of the Study:
- To identify long non-coding RNAs (lncRNAs) involved in cell division using a large-scale RNAi screen.
- To investigate the function of the lncRNA linc00899 in mitosis and its molecular targets.
Main Methods:
- High-content RNA interference (RNAi) imaging screen of over 2,000 human lncRNAs.
- Transcriptome analysis of cells depleted of linc00899.
- Investigation of linc00899's interaction with and regulation of TPPP/p25.
Main Results:
- Depletion of linc00899 caused a significant mitotic delay in various cell types.
- Transcriptome analysis identified the neuronal microtubule-binding protein TPPP/p25 as a target of linc00899.
- linc00899 directly binds to TPPP/p25 and suppresses its transcription, leading to altered microtubule dynamics and mitotic delay when depleted.
Conclusions:
- The lncRNA linc00899 plays a crucial role in regulating mitotic progression and genome stability.
- linc00899 controls microtubule behavior by modulating TPPP/p25 expression, with implications extending beyond cell division.
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