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Cu∥-loaded polydopamine coatings with in situ nitric oxide generation function for improved hemocompatibility.
Lei Zhou1, Xin Li1, Kebing Wang1
1Key Laboratories of Advanced Technology for Materials of Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
Regenerative Biomaterials
|April 17, 2020
Summary
Copper-loaded polydopamine coatings promote nitric oxide (NO) release, crucial for cardiovascular health. These novel materials demonstrate excellent hemocompatibility, inhibiting thrombosis and platelet activation for advanced blood contact applications.
Area of Science:
- Biomaterials Science
- Cardiovascular Research
- Nanotechnology
Background:
- Nitric oxide (NO) is a vital signaling molecule produced by endothelial cells, regulating cardiovascular homeostasis, vasodilation, and preventing atherosclerosis.
- Insufficient NO release is linked to pathological conditions like atherosclerosis, highlighting the need for therapeutic strategies.
- Endogenous NO donors can be stimulated to release NO, offering potential treatments for blood system diseases.
Purpose of the Study:
- To develop and characterize novel copper-loaded polydopamine (PDA) coatings.
- To investigate the NO-releasing capabilities of these coatings.
- To evaluate the hemocompatibility and antithrombotic properties of the developed materials.
Main Methods:
- Synthesis of copper-loaded PDA coatings via self-polymerization for varying durations (24, 48, 72 h).
- Characterization using phenol hydroxyl quantification, X-ray photoelectron spectroscopy, ellipsometry, atomic force microscopy, and water contact angle measurements.
- Assessment of glutathione peroxidase-like activity and NO release from S-nitrosoglutathione (GSNO).
- Evaluation of hemocompatibility, including platelet adhesion, activation, and thrombosis formation.
Main Results:
- Coating thickness and surface phenolic hydroxyl density increased with polymerization time.
- Copper-loaded PDA coatings exhibited glutathione peroxidase-like activity, catalyzing NO release from GSNO.
- Surfaces demonstrated favorable hemocompatibility, inhibiting platelet adhesion and activation.
- Thrombosis formation was significantly suppressed on the copper-loaded coatings.
Conclusions:
- Copper-loaded PDA coatings can effectively catalyze NO release.
- These coatings possess excellent hemocompatibility and antithrombotic properties.
- The developed materials offer a promising platform for advanced blood-contacting applications.
