Chryseobacterium salivictor sp. nov., a plant-growth-promoting bacterium isolated from freshwater

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated NBC 122^T, was isolated from freshwater of the Nakdong River Republic of Korea. Growth occurred at pH 5.0-8.0 (optimum, pH 7.0), at 4-37 °C (optimum, 25 °C), and with 0-3.5% (w/v) NaCl (optimum, 1%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain NBC 122^T belongs to the genus Chryseobacterium and is most closely related to Chryseobacterium antarcticum AT1013^T (97.85%). The average nucleotide identity and in silico DNA-DNA hybridization (DDH) values between strain NBC 122^T and related Chryseobacterium species were 77.77-80.28 and 20.9-23.2%, respectively. The strain NBC 122^T contained MK-6 as the major respiratory quinone. The major fatty acids included anteiso C_15:0, iso C_15:0, summed feature 9 (C_17:1 iso ω9c, C_16:0 10-methyl) and iso C_17:0 3-OH, and the polar lipids were phosphatidylethanolamine, five unidentified aminolipids and six unidentified lipids. The DNA G + C content was 37.5 mol%. On the basis of a 16S rRNA gene phylogeny and comparative phenotypic analyses, strain NBC 122^T represents a novel species in the genus Chryseobacterium, for which the name Chryseobacterium salivictor sp. nov. is proposed. The type strain is NBC122^T (= KCTC 72248^T = JCM 33980^T).