Comparative evaluation of ⁶⁸Ga-labelled TATEs: the impact of chelators on imaging

⁶⁸Ga-labelled peptides targeting somatostatin receptor 2 (SSTR2) have demonstrated encouraging results in managing patients with neuroendocrine tumours (NETs). In addition to metal chelation, bifunctional chelators have also been found to impact imaging outcomes due to their differences in stability, charge, hydrophilicity, etc. In the present work, a comparative pharmacokinetic evaluation and imaging characteristics were performed between ⁶⁸Ga-labelled somatostatin analogues (TATE) using NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid) and DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) as bifunctional chelating agents (BFCAs).

Both ⁶⁸Ga-NOTA-TATE and ⁶⁸Ga-DOTA-TATE were obtained with high radiochemical purity. ⁶⁸Ga-NOTA-TATE demonstrated higher in vitro stability (≥ 99%) than ⁶⁸Ga-DOTA-TATE (≥ 95%) after 3 h of incubation. The water solubilities (partition coefficients, - 1.76 ± 0.06 vs. - 2.72 ± 0.16) and plasma protein binding rates (12.12% vs. 30.6%) were lower for ⁶⁸Ga-NOTA-TATE than for ⁶⁸Ga-DOTA-TATE. Differential pharmacokinetics and comparable tumour affinities (within 1 h) were observed in AR42J tumour-bearing mice. Healthy volunteer imaging studies showed comparable distribution patterns of these two imaging agents. However, the maximum standardized uptake values (SUVmax) of the two tracers varied in each organ. The two PET agents demonstrated almost identical SUVmax values in the kidneys. ⁶⁸Ga-NOTA-TATE did have a lower SUVmax in most other organs compared with ⁶⁸Ga-DOTA-TATE, including the liver (4.2 vs. 10.1), potentially due to the lower protein binding rate.

⁶⁸Ga-NOTA-TATE and ⁶⁸Ga-DOTA-TATE demonstrated comparable tumour uptake in an AR42J mouse model. An initial clinical study revealed that ⁶⁸Ga-NOTA-TATE may have reduced background uptake in the major organs such as the liver. Although the subject numbers were limited, further investigation of ⁶⁸Ga-NOTA-TATE is warranted for detecting SSTR2-positive neuroendocrine tumours.