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The melanoma progression-associated antigen P3.58 is identical to the intercellular adhesion molecule, ICAM-1
J P Johnson1, B G Stade, U Hupke
1Institute of Immunology, Munich, Federal Republic of Germany.
Abstract:
The 89kd cell surface glycoprotein, P3.58, is expressed on human malignant melanomas in situ where it is associated with an increased risk of metastatic disease. Monoclonal antibodies detecting denatured P3.58 were produced and used to isolate a P3.58 encoding cDNA clone from a human melanoma lambda expression library. Sequencing of the cDNA revealed that the P3.58 antigen is identical to the leukocyte intercellular adhesion molecule 1 (ICAM-1).
Insights
The P3.58 cell surface glycoprotein, linked to melanoma metastasis risk, was identified. It is identical to leukocyte intercellular adhesion molecule 1 (ICAM-1).
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- The P3.58 glycoprotein is an 89kd cell surface marker found on human malignant melanomas.
- Expression of P3.58 is correlated with a higher risk of metastatic melanoma.
Purpose of the Study:
- To characterize the P3.58 antigen.
- To identify the molecular identity of P3.58.
Main Methods:
- Production of monoclonal antibodies against denatured P3.58.
- Isolation of a P3.58 encoding cDNA clone using a human melanoma lambda expression library.
- Sequencing of the isolated cDNA.
Main Results:
- Monoclonal antibodies were successfully generated to detect denatured P3.58.
- A cDNA clone encoding P3.58 was isolated from a melanoma expression library.
- Sequence analysis confirmed that P3.58 is identical to leukocyte intercellular adhesion molecule 1 (ICAM-1).
Conclusions:
- The P3.58 antigen, associated with melanoma metastasis, is leukocyte intercellular adhesion molecule 1 (ICAM-1).
- This finding provides a molecular target for understanding melanoma progression and potential therapeutic strategies.