Related Experiment Video

Updated: Dec 24, 2025

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma
10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

676

Long non-coding RNA DDX11-AS1 promotes non-small cell lung cancer development via regulating PI3K/AKT signalling

Xuegang Feng1, Shengsheng Yang1, Shunkai Zhou1

  • 1Department of Cardiothoracic Surgery, 900th Hospital of Joint Logistics Support Force of People's Liberation Army, Fuzhou, China.

Clinical and Experimental Pharmacology & Physiology
|April 17, 2020
PubMed

Insights

The long non-coding RNA DDX11-AS1 promotes non-small cell lung cancer (NSCLC) development. Inhibiting DDX11-AS1 suppresses NSCLC cell proliferation and enhances apoptosis by affecting the PI3K/AKT pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is the most common form of lung cancer.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific role of DDX11-AS1 in NSCLC progression remained largely uncharacterized.

Purpose of the Study:

  • To investigate the biological role of the lncRNA DDX11-AS1 in NSCLC.
  • To elucidate the molecular mechanism underlying DDX11-AS1's function in NSCLC progression.
  • To determine if DDX11-AS1 influences the PI3K/AKT signaling pathway.

Main Methods:

  • Bioinformatics analysis and experimental assays including H&E staining, RT-qPCR, colony formation, CCK-8, flow cytometry, western blot, and xenograft assays.
  • Assessing DDX11-AS1 expression levels in NSCLC tissues and cells.
  • Evaluating the functional impact of DDX11-AS1 knockdown on NSCLC cell proliferation, apoptosis, and in vivo tumor growth.
  • Investigating the effect of DDX11-AS1 on the PI3K/AKT signaling pathway.

Main Results:

  • DDX11-AS1 expression was significantly upregulated in NSCLC tumor tissues and cells.
  • Knockdown of DDX11-AS1 inhibited NSCLC cell proliferation both in vitro and in vivo.
  • Inhibition of DDX11-AS1 promoted apoptosis in NSCLC cells.
  • DDX11-AS1 regulates the phosphorylation of AKT, indicating activation of the PI3K/AKT signaling pathway.

Conclusions:

  • DDX11-AS1 acts as an oncogenic lncRNA that promotes the progression of NSCLC.
  • DDX11-AS1 exacerbates NSCLC development by activating the PI3K/AKT signaling pathway.
  • Targeting DDX11-AS1 may represent a potential therapeutic strategy for NSCLC.
Keywords:
DDX11-AS1NSCLCPI3K/AKT signalling

More Related Videos

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.6K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K