Nitrate reduction by Paracoccus thiophilus strain LSL 251 under aerobic condition: Performance and intracellular
Shengnan Chen1, Sulin Li1, Tinglin Huang1
1Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Municipal and Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Abstract:
The intracellular carbon metabolic flux pathways of denitrifying bacteria under aerobic conditions remain unclear. Here, a newly strain LSL251 was identified as Paracoccus thiophilus. Strain LSL251 removed 94.79% and 98.78% of total organic carbon and nitrate. 74.66% of nitrogen in culture system was lost as gaseous nitrogen. Moreover, 13C stable isotopic labeling and metabolic flux analyses revealed that the primary intracellular carbon metabolic pathways were the Entner-Doudoroff pathway and the tricarboxylic acid (TCA) cycle. Electrons are primarily donated as direct electron donor-NADH through the TCA cycle. Furthermore, response surface methodology modeled that the highest total nitrogen removal efficiency was 98.43%, where the optimal parameters were C/N ratio of 8.00, 32.98 °C, 50.18 rpm, and initial pH of 7.73. All together, these results have shed new lights on intracellular central carbon metabolic distribution and flux pathways of aerobic denitrifying bacteria.
More Related Videos
07:26Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
10:23Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
Related Concept Videos
Inorganic Nitrogen Assimilation
Metabolism of Chemolithotrophs
Carbon-dioxide Fixation
Microbial Nutrition
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Respiration Pathways
