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Updated: Dec 23, 2025

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

Improving extracellular vesicles visualization: From static to motion.

Pablo Reclusa¹, Peter Verstraelen², Simona Taverna³

¹Center for Oncological Research (CORE), University of Antwerp, Antwerp, Belgium.

Scientific Reports

|April 18, 2020

Summary

This summary is machine-generated.

Extracellular vesicles (EVs) are crucial in cancer progression and drug delivery. Researchers developed a new method to track labeled EVs, visualizing their uptake by living lung cancer cells for the first time.

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Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Area of Science:

Extracellular vesicle (EV) research
Cancer biology
Nanomedicine

Background:

Extracellular vesicles (EVs) are increasingly recognized for their roles in cancer progression, including metastasis.
EVs mediate intercellular communication and can be internalized by cells via integrins in a tissue-specific manner.
The potential of EVs as drug delivery vehicles in cancer therapy is significant, but requires improved isolation and visualization methods.

Purpose of the Study:

To develop and validate an improved protocol for tracking extracellular vesicles (EVs).
To enable in vivo analysis of EV internalization in cancer cells.
To visualize the uptake of labeled EVs by living lung cancer cells.

Main Methods:

Development of a novel protocol for labeling and tracking extracellular vesicles (EVs).
Utilizing advanced imaging techniques to observe EV-cell interactions in real-time.
Focusing on the internalization of labeled EVs by living lung cancer cells.

Main Results:

Successful development of a protocol for tracking labeled EVs in vivo.
First-ever visualization of labeled EVs being taken up by living lung cancer cells.
Demonstration of EVs' internalization mechanism in lung cancer models.

Conclusions:

The developed protocol significantly advances the ability to study EV dynamics in cancer.
This breakthrough enables better understanding of EV-mediated processes in cancer progression and drug delivery.
Future applications in cancer diagnostics and therapeutics are promising.