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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Published on: May 16, 2022

Surface plasmon-coupled emission imaging for biological applications.

Min Chen¹, Shuo-Hui Cao¹, Yao-Qun Li²

¹Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, Fujian, China.

Analytical and Bioanalytical Chemistry

|April 18, 2020

Summary

Surface plasmon-coupled emission imaging (SPCEi) enhances axial resolution in fluorescence microscopy. This technique offers improved sensitivity and confinement for biological research and medical diagnostics.

Keywords:

Background suppression Biological application Cell imaging Directional emission Fluorescence microscopy Surface plasmon–coupled emission imaging

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Area of Science:

Optical Imaging
Nanotechnology
Biophysics

Background:

Fluorescence imaging is vital in chemical and biological research due to its sensitivity and specificity.
Improving spatial resolution, particularly axial resolution, remains a significant challenge in microscopy.
Existing methods for enhancing axial resolution are often complex and not widely accessible.

Purpose of the Study:

To provide an overview of Surface Plasmon-Coupled Emission Imaging (SPCEi) development.
To describe the optical characteristics and system constructions of SPCEi.
To highlight recent advancements and future prospects of SPCEi in biological applications.

Main Methods:

Utilizing surface plasmon-coupled emission (SPCE) generated by fluorophore-metal film interactions.
Developing SPCE imaging (SPCEi) systems with unique optical properties.
Reviewing and analyzing recent biological applications of SPCEi.

Main Results:

SPCEi offers improved sensitivity and axial confinement compared to conventional fluorescence imaging.
The technique leverages the directional and distance-dependent nature of SPCE.
SPCEi demonstrates excellent performance in bioimaging applications.

Conclusions:

SPCEi presents a promising platform for high-resolution optical imaging.
The technology has potential for widespread applications in biological research and medical diagnostics.
Further development of SPCEi could overcome current limitations in axial resolution.