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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Dec 23, 2025

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Published on: September 16, 2019

A method for isolating RNA from canine bone.

Rebecca Nance^1,2, Payal Agarwal^1,2, Maninder Sandey²

¹Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.

|April 18, 2020

Summary

This summary is machine-generated.

This study presents a new method for isolating high-quality RNA from canine bone cells. This technique enables accurate gene expression analysis through transcriptomic sequencing.

Keywords:

RNA bone canine dog osteoblasts osteoclasts osteocytes sequencing

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Last Updated: Dec 23, 2025

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Published on: September 16, 2019

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Published on: December 18, 2019

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

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Published on: May 19, 2019

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

RNA extraction from bone is crucial for gene expression studies like transcriptomic sequencing.
Bone's mineralized and hypocellular nature complicates tissue homogenization and yields low RNA amounts.
Contaminant tissue removal is vital for isolating bone cell-specific RNA.

Purpose of the Study:

To establish an effective method for isolating RNA from normal canine bone cells.
To ensure RNA purity, free from contaminating tissues.
To prepare samples for downstream transcriptomic analysis.

Main Methods:

Utilized canine phalanges for RNA extraction.
Developed a protocol to overcome challenges of bone homogenization.
Implemented steps for removing non-bone cell tissues.

Main Results:

Successfully isolated sufficient quantity and quality RNA from canine bone cells.
Achieved RNA isolation without contamination from surrounding tissues.
Demonstrated the method's suitability for transcriptomic analysis.

Conclusions:

The established method effectively isolates pure RNA from canine bone.
This technique supports accurate gene expression profiling in bone cells.
The protocol is valuable for transcriptomic studies using bone samples.