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Gold alloy-based nanozyme sensor arrays for biothiol detection.

Junshu Lin1, Quan Wang, Xiaoyu Wang

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This study introduces novel gold alloy nanozymes for detecting and differentiating various biothiols. The developed nanozyme sensor arrays offer a cost-effective and convenient method for analyzing these important molecules in biological samples like serum.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Biothiols are crucial in cellular functions and disease pathogenesis.
  • Existing biothiol detection methods lack cost-effectiveness, convenience, and differentiation capabilities.
  • Nanozymes offer advantages like stability and multifunctionality for biosensing applications.

Purpose of the Study:

  • To develop a facile, cost-effective sensor array for biothiol detection and differentiation.
  • To synthesize novel gold alloy nanozymes with enhanced peroxidase-like activity.
  • To demonstrate the differentiation of six common biothiols using a cross-reactive sensor array.

Main Methods:

  • Synthesis of gold alloy nanozymes with superior peroxidase-like activity compared to gold nanoparticles.
  • Construction of cross-reactive sensor arrays utilizing three distinct alloy nanozymes.
  • Colorimetric detection assay based on the competitive inhibition of nanozyme activity by biothiols.

Main Results:

  • Successfully detected and discriminated six typical biothiols: glutathione, cysteine, dithiothreitol, mercaptoacetic acid, mercaptoethanol, and mercaptosuccinic acid.
  • The nanozyme sensor arrays exhibited enhanced peroxidase-like activities.
  • Demonstrated successful application in discriminating biothiols within human serum samples.

Conclusions:

  • The developed gold alloy nanozyme sensor arrays provide a sensitive and selective platform for biothiol detection and differentiation.
  • This approach offers a promising tool for clinical diagnostics and biochemical analysis.
  • The study highlights the potential of nanozymes in creating advanced biosensing systems.