Colorimetric and fluorescence sensing of Zn²⁺ ion and its bio-imaging applications based on macrocyclic "tet a"...

We report on the synthesis and characterization of trans N, N'-di-substituted macrocyclic "tet a" probe (L) for metal ion sensing. Both the colorimetric and fluorescent titration studies are performed with different metal ions. The results have suggested that the probe L is very selective and sensitive towards Zn²⁺ ions with significant changes in color. The pendant armed macrocyclic "tet a" probe has exhibited 1.28× 10⁵ M^-1 binding constant and virtuous selectivity for Zn²⁺ ion than other common metal ions. The detection limit of the probe towards Zn²⁺ ion is 0.027 nM. The selective sensing of Zn²⁺ ion is efficiently reversible with EDTA, which is demonstrated for five cycles without losing sensitivity. The time-resolved single-photon counting (TCSPC) studies have determined the average lifetime value for the probe L and L+ Zn²⁺ ion of 1.29 and 2.96 ns, respectively. The theoretical DFT studies have well supported the experimental outcomes. The practical application of the probe in visualizing intracellular Zn²⁺ ion distribution in live Artemia salina has proved the low cytotoxicity and cell membrane permeability of probe, which makes it capable of sensing Zn²⁺ ion in HeLa cells. Thus, the probe L can act as a selective recognition of Zn²⁺ ion in living cell applications.