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Identifying gram-positive cocci in dermatoscopes and smartphone adapters using MALDI-TOF MS: a cross-sectional study.

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  • 1Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Department of Dermatology, Hospital Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.

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Summary

Dermatoscopes frequently harbor gram-positive cocci, particularly coagulase-negative staphylococci, posing a potential infection risk. Hospital settings and intensive care units are associated with higher bacterial contamination on dermatoscopes.

Keywords:
DermoscopyGram-positive cocciMass spectrometryMicrobial sensitivity tests

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Area of Science:

  • Dermatology
  • Microbiology
  • Infection Control

Background:

  • Dermatoscope use is increasing, raising concerns about potential microorganism transmission between healthcare providers and patients.
  • Understanding bacterial contamination on dermatoscopes is crucial for patient safety and infection prevention protocols.

Purpose of the Study:

  • To identify common gram-positive cocci on dermatoscopes and smartphone adapters.
  • To determine the antibiotic resistance profiles of identified bacteria.
  • To evaluate factors linked to increased dermatoscope bacterial contamination.

Main Methods:

  • A cross-sectional study involving 118 dermatologists in Brazil.
  • Bacterial identification using MALDI-TOF MS.
  • Assessment of dermatoscope usage habits via an anonymous questionnaire.

Main Results:

  • Gram-positive cocci were found on 46.6% of dermatoscope lenses and 37.3% of on/off buttons.
  • Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus warneri were the most prevalent species.
  • Hospital-based use, particularly with inpatients and in intensive care units, correlated with higher contamination.
  • High resistance rates were noted for penicillin, erythromycin, and sulfamethoxazole-trimethoprim.

Conclusions:

  • Coagulase-negative staphylococci are frequently detected on dermatoscopes.
  • Staphylococcus aureus was identified solely on the lens.
  • Further research is needed to investigate gram-negative bacilli, fungi, and viruses, and to analyze a larger number of adapters.