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Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Dec 23, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Improved soybean transformation for efficient and high throughput transgenic production.

Dayakar Pareddy¹, Siva Chennareddy², Geny Anthony²

¹Dow AgroSciences LLC, Trait Product Development, Indianapolis, IN, USA.

Transgenic Research

|April 19, 2020

Summary

Researchers developed a new soybean transformation method using organogenesis and Agrobacterium. This efficient technique achieves high transformation frequencies, enabling faster development of genetically modified soybean varieties.

Keywords:

Agrobacterium tumefaciens EHA105 Glufosinate selection Glycine max L.Soybean Transformation

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Last Updated: Dec 23, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

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Published on: May 5, 2023

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

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Published on: September 27, 2016

Area of Science:

Plant Biotechnology
Genetics
Molecular Biology

Background:

Genetic transformation of soybean has been established for over 20 years but remains inefficient.
Improving soybean transformation efficiency is crucial for both basic research and commercial applications.

Purpose of the Study:

To develop an efficient organogenesis-based genetic transformation method for soybean.
To optimize Agrobacterium-mediated transformation using specific explants and strains.

Main Methods:

Utilized a split-seed explant with an attached partial embryonic axis from imbibed soybean seeds.
Employed Agrobacterium tumefaciens strain EHA105 for transformation.
Used phosphinothricin acetyl transferase for glufosinate selection of transgenic events.

Main Results:

Achieved an average transformation frequency of 18.7%.
Agrobacterium strain EHA105 yielded higher transformation frequencies and more low-copy events than EHA101.
Demonstrated 48% heritability of transgenes in T1 progeny through molecular analysis and herbicide screening.
The method proved applicable across different soybean genotypes, with elite lines showing high efficiency.

Conclusions:

The developed split-seed system with a partial embryonic axis significantly enhances soybean transformation efficiency.
This method facilitates high-throughput production of transgenic soybean events for research and commercial development.
Optimized Agrobacterium strains and explant preparation are critical for successful soybean genetic modification.