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Updated: Aug 4, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
Metazoan Soluble β-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of Their
Guillaume St-Pierre1, Ann Rancourt1, Sachiko Sato2
1Glycobiology and Bioimaging Laboratory, Research Centre for Infectious Diseases, Faculty of Medicine, Laval University and GlycoNET, Quebec, Canada.
Abstract:
Galectins are a family of soluble β-galactoside-binding proteins that share conserved carbohydrate recognition domain. Galectins are found in most multicellular organisms and exert various biological functions by binding to the surface glycoconjugates as lectins. In this chapter, we describe the general methods of purification of galectins, quality control of purified galectins, some example methods of evaluating their carbohydrate-binding activity, and use of galectin to search or detect galectin ligands as well as a series of precautions for the usage of galectins.
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