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Updated: Dec 23, 2025
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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
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Purification of GNA-Related Lectins from Natural Sources
1Laboratory of Glycobiology and Biochemistry, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium. ElsJM.VanDamme@UGent.be.
Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2020
Abstract:
The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.
