Pleurotus cornucopiae Mycelial Lectin (PCL-M): Purification and Detection of the Activity on Mycelial Surface

Pleurotus cornucopiae mycelial lectin (PCL-M) is a divalent cation-dependent GalNAc-specific lectin that is purified from the mycelia of the edible mushroom Pleurotus cornucopiae. PCL-M is produced only in dikaryotic mycelia growing on solid medium and is not present in the mycelia of fruiting bodies. It is a multimeric glycoprotein composed of 40 kDa subunits linked by disulfide bonds. PCL-M is localized on the surface of aggregating mycelia and may be involved in primordia formation. The procedure for PCL-M purification has been discussed herein. Additionally, an experimental method for analyzing the localization of PCL-M on mycelial surface has been described.