Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

493
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
493
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

407
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
407
Methods of Classification and Identification01:28

Methods of Classification and Identification

825
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
825

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flying Droplet Sensor Based on H-GQDs/CNF Network Structure and Its Applications.

ACS sensors·2026
Same author

Simple Hydrodynamic Molecular Weight Model for Rapid Assessment of Therapeutic Protein Oligomerization States in Formulation.

The AAPS journal·2026
Same author

Pollutant-specific carbon footprint analysis and decarbonization potential for municipal wastewater: A case study in Xi'an, China.

Journal of environmental management·2026
Same author

A multi-condition acoustic dataset of ball bearings for fault diagnosis.

Data in brief·2026
Same author

Optical communication based on tunable intensity vortex arrays.

Optics express·2026
Same author

Association between metabolic heterogeneity of obesity and biological aging among adults: a population-based study.

The American journal of the medical sciences·2026

Related Experiment Video

Updated: Dec 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

30.9K

Identifying Potential Polymicrobial Pathogens: Moving Beyond Differential Abundance to Driver Taxa.

Jiaqi Lu1,2, Xuechen Zhang1,2, Qiongfen Qiu2

  • 1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China.

Microbial Ecology
|April 21, 2020
PubMed
Summary

This study identifies key Vibrio species as primary colonizers in shrimp white feces syndrome (WFS), revealing their role in disease progression and enabling the design of targeted probiotics for aquatic animal health.

Keywords:
Driver taxaGut microbiotaPolymicrobial pathogensPrimary colonizerShrimp disease

More Related Videos

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

19.7K
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.3K

Related Experiment Videos

Last Updated: Dec 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

30.9K
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

19.7K
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.3K

Area of Science:

  • Aquatic animal pathology
  • Microbial ecology
  • Disease diagnostics

Background:

  • Aquatic animal diseases are often caused by polymicrobial infections, challenging the "one pathogen, one disease" model.
  • Accurate identification of multiple pathogens is crucial for developing effective probiotic treatments.

Purpose of the Study:

  • To investigate polymicrobial pathogens associated with shrimp white feces syndrome (WFS) based on their abundance changes during disease progression.
  • To identify key microbial "drivers" and potential probiotic targets in WFS.

Main Methods:

  • Analysis of microbial community composition and abundance changes over WFS progression.
  • Application of Random Forest and NetShift models to identify disease-associated pathogens and their ecological roles.
  • Prediction of functional pathways affected in diseased shrimp.

Main Results:

  • Three Vibrio species (V. fluvialis, V. coralliilyticus, V. tubiashii) were identified as primary colonizers driving WFS.
  • These primary colonizers accurately diagnosed shrimp health status (91.4% accuracy) and acted as "drivers" in microbiota shifts.
  • Commensal bacteria antagonizing these Vibrio species were identified, and key metabolic pathways were found to be downregulated in diseased shrimp.

Conclusions:

  • An ecological framework was established to infer polymicrobial pathogens and design targeted probiotics by quantifying microbial "driver" features.
  • This approach offers a method for understanding disease etiology in aquatic animals and developing novel therapeutic strategies.