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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

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Published on: May 1, 2015

Lymphatic MAFB regulates vascular patterning during developmental and pathological lymphangiogenesis.

Lothar C Dieterich¹, Carlotta Tacconi¹, Franziska Menzi¹

¹Institute of Pharmaceutical Sciences, ETH Zurich, 8093, Zurich, Switzerland.

|April 21, 2020

Summary

MAFB, a transcription factor, plays a role in lymphatic vessel development. Its absence in lymphatic endothelial cells impacts embryonic development and enhances tumor lymphangiogenesis, suggesting therapeutic potential.

Keywords:

Branching Lymphangiogenesis Postnatal development Transcription factor Vascular morphogenesis

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Published on: May 20, 2011

Area of Science:

Vascular biology
Developmental biology
Molecular genetics

Background:

MAFB is a transcription factor crucial for cell differentiation, including macrophages and keratinocytes.
MAFB is expressed in lymphatic endothelial cells (LECs) and regulated by VEGF-C/VEGFR-3 signaling.
Previous studies indicated MAFB regulates lymphatic differentiation genes and its global knockout affects embryonic lymphatic patterning.

Purpose of the Study:

To investigate the role of MAFB in LEC-intrinsic lymphatic development.
To determine MAFB's involvement in postnatal lymphangiogenesis and pathological conditions.

Main Methods:

Generation of conditional, lymphatic-specific Mafb knockout mice.
Analysis of lymphatic vessel patterning during embryogenesis and postnatal development.
Assessment of lymphangiogenesis in healthy and tumor-bearing adult mice.

Main Results:

Conditional knockout mice exhibited lymphatic patterning defects similar to global knockout embryos.
MAFB deficiency in LECs led to increased lymphatic branching in the diaphragm at P7.
Absence of MAFB enhanced tumor-induced lymphangiogenesis but had minimal impact on adult lymphatic function.

Conclusions:

LEC-expressed MAFB is essential for embryonic lymphatic vascular morphogenesis.
MAFB influences postnatal lymphatic development and is a key regulator of pathological lymphangiogenesis.
MAFB presents a potential therapeutic target for modulating lymphangiogenesis.