Depletion of langerin⁺ cells enhances cutaneous wound healing

Langerin is a C-type lectin receptor that is expressed on Langerhans cells and langerin-positive dermal dendritic cells in the skin. Little is known about the function of langerin⁺ cells in wound healing. In this study, the effects of ablation of langerin⁺ cells on healing of a full-thickness excision wound were investigated using the langerin-DTR depletable mouse. Strikingly, depletion of langerin⁺ cells resulted in more rapid reduction in wound area. Accelerated wound healing in the langerin⁺ -cell-depleted group was characterized by enhanced neo-epidermis and granulation tissue formation, and increased cellular proliferation within the newly formed tissues. Accelerated healing in the absence of langerin⁺ cells was associated with increased levels of granulocyte-macrophage colony-stimulating factor, F4/80⁺ cells and blood vessels within the granulation tissue. These data support an inhibitory role for langerin⁺ cells during wound healing. Therapies that suppress langerin⁺ cells or their function may therefore have utility in progressing the healing of wounds in humans.