Related Experiment Video
Updated: Dec 23, 2025
12:34
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
13.9K
A database for inventory of proteoform profiles: "2DE-pattern"
Stanislav Naryzhny1,2, Nikolay Klopov2, Natalia Ronzhina2
1Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, Moscow, Russia.
Electrophoresis
|April 21, 2020
Summary
A new database, "2DE-pattern," inventories human proteoforms identified by two-dimensional electrophoresis (2DE) and mass spectrometry. This resource aids researchers in navigating complex proteomic data and understanding protein diversity.
Area of Science:
- Proteomics
- Bioinformatics
- Molecular Biology
Background:
- The human proteome comprises diverse gene products/proteoforms.
- Managing proteoform data from various methods is challenging.
Purpose of the Study:
- To present a novel database for organizing proteoform information.
- To provide a centralized resource for proteomic data inventory.
Main Methods:
- Separation of proteoforms using two-dimensional electrophoresis (2DE).
- Analysis using shotgun electrospray ionization-liquid chromatography-tandem mass spectrometry (ESI-LC-MS/MS).
Main Results:
- Development of the "2DE-pattern" database.
- The database stores isoform-centric patterns of proteoforms.
Conclusions:
- The "2DE-pattern" database offers a structured approach to proteomic data management.
- This resource facilitates research on human proteoform diversity.
