Related Experiment Videos
[Malate dehydrogenase isoenzymes in myocardial infarction]
Insights
Cardiac enzyme levels in myocardial infarction patients reveal disease severity. Creatine phosphokinase (CPK) and malate dehydrogenase (MDH) peaked earlier than lactate dehydrogenase (LDH) and aspartate aminotransferase (AST).
Area of Science:
- Biochemistry
- Cardiology
- Clinical Diagnostics
Background:
- Myocardial infarction (MI) diagnosis relies on cardiac enzyme monitoring.
- Understanding enzyme kinetics aids in assessing MI severity and prognosis.
Purpose of the Study:
- To correlate plasma enzyme activities (CPK, MDH, AST, LDH) with MI severity and time since onset.
- To evaluate the diagnostic and prognostic value of specific enzyme isoenzymes.
Main Methods:
- Assessed plasma CPK, MDH, AST, and LDH isoenzyme activities in 60 MI patients.
- Correlated enzyme peak times and normalization rates with disease severity and clinical outcomes.
Main Results:
- CPK and MDH-C activities peaked earlier than LDH-M and AST-C.
- Higher MI severity correlated with delayed enzyme peak onset and slower activity decrease.
- Elevated mitochondrial isoenzymes and LDH-M indicated greater disease severity.
- Delayed normalization of enzyme activities predicted a poorer prognosis.
Conclusions:
- Enzyme activity profiles, particularly peak times and normalization rates, offer insights into MI severity.
- Mitochondrial isoenzymes and LDH-M are valuable indicators of MI severity.
- Delayed enzyme normalization is linked to adverse outcomes in myocardial infarction patients.
Abstract:
Plasma CPK activity and the activity of isoenzymes MDH, AST and LDH were assessed in 60 patients with myocardial infarction of different severity, with reference to the time since the onset of the attack. The peaks of CPK and MDH-C activity were reached sooner than those of LDH-M and AST-C, while the CPK and MDH-C curves were similar. The severity of the disease showed correlation to later onset of enzyme peaks and markedly delayed decrease in the respective values. Increased activity of mitochondrial isoenzymes and blood LDH-M provided additional information on the severity of the disease. Delayed normalization of these activities was associated with a poor diagnosis.