Related Experiment Video
Updated: Dec 23, 2025

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
Root Coverage with Platelet-Rich Fibrin in Miller's Class I, III, and IV Gingival Retractions
David A Gutiérrez1, Juan Pablo Hinojosa1, Juan P Pava1
1Faculty of Dentistry, Research Group in Buccal Innovation. Universidad Antonio Nariño, Popayán, Cauca, Colombia.
Abstract:
Coronally advanced flap (CAF) with connective tissue graft (CTG) is considered as the most predictable method to treat Miller's Class I and II gingival recessions (GR). However, due to the patient's postsurgical discomfort, the difficulty of finding a donor with an adequate tissue thickness, the high costs of acellular dermal substitute among others, the platelet concentrates have been found to scaffold alternative to replace CTG as a part of the CAF treatment for GR. Nevertheless, according to the recent literature, the evidence of success in Class III and IV has been limited for CAF and platelet-rich fibrin (PRF). The purpose of this report is to present a case of multiple Miller's Class III and IV GR treated with CAF and PRF where the potential of PRF to increase gingival thickness and clinical attachment level, and improve soft-tissue healing and clinical appearance was corroborated.
Related Concept Videos
Clot Retraction and Fibrinolysis
Formation of the Platelet Plug
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

