Closure of rabbit ileum enterotomies with the argon and CO2 lasers: bursting pressures and histology

In this study, we examined short-term strength and histology of experimental argon and CO2 laser-welded and control-sutured enterotomies in a rabbit model. Longitudinal 1.0-cm enterotomies were closed with the argon laser at 0.5 (n = 10) or 1.0 (n = 10) W power and a spot diameter of 2.8 mm resulting in energy fluences of 230 and 450 J/cm2, respectively. Similar enterotomies were closed using the CO2 laser at 0.5 (n = 10) or 1.0 (n = 10) W power and a spot diameter of 1.2 mm, resulting in energy fluences of 1,360 and 2,730 J/cm2. In all closures, continuous wave laser was delivered for 30 seconds. Using a pressure-monitored infusion system with normal saline, the pressure required to burst each weld as well as sutured controls (n = 10) was recorded approximately 1 minute following fusion. Mean bursting pressures for the argon laser at 0.5 W and 1.0 W were 34.1 +/- 19.4 mm Hg (mean +/- SD) and 17.3 +/- 8.3 mm Hg, respectively, and for the CO2 laser were 23.5 +/- 12.0 mm Hg and 31.8 +/- 15.6 mm Hg, respectively. Sutured controls leaked at 45.2 +/- 12.0 mm Hg. With the exception of argon-laser welds at an energy fluence of 450 J/cm2, which were less than sutured repairs, the bursting pressures for welded closures were not significantly different from the sutured controls (Student's t distribution, P less than .05). Histologic examination of both types of welds demonstrated a fibrin and red blood cell coagulum bridging the anastomosis, with some evidence of mild thermal injury at the mucosa.(ABSTRACT TRUNCATED AT 250 WORDS)