Related Experiment Video
Updated: Dec 23, 2025
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Efficacy of three disinfectant formulations and a hydrogen peroxide/silver fogging system on surfaces experimentally
Julie Soohoo1, Joshua B Daniels2, Stephanie A Brault1
1Department of Clinical Sciences, Colorado State University College of Veterinary Medicine and Biomedical Sciences, 300 West Drake Road, Fort Collins, CO, 80523, USA.
Background:
Effective environmental disinfection is necessary to prevent nosocomial infections from meticillin-resistant Staphylococcus pseudintermedius (MRSP). However, there are currently no commercial disinfectant sprays or fogging systems with label claims against MRSP.
Hypothesis/Objectives:
To evaluate the efficacy of a quaternary ammonium product (QAC), an accelerated hydrogen peroxide product (AHP), a hydrogen peroxide and silver product (HAL), and a hydrogen peroxide and silver fogging system (FOG) against MRSP.
Methods And Materials:
Sterile plastic surfaces inoculated with MRSP were treated with 200 µL of QAC, AHP or HAL for the recommended contact times. For FOG, inoculated samples were placed in eight positions within a sealed room before fogging for the recommended contact time. Post-treatment bacterial counts were compared to untreated positive controls. Sterile uninoculated surfaces served as negative controls.
Results:
Least-squares mean reduction (log10 ) in colony forming units (cfu) was 3.55 log10 for QAC (P < 0.0001), 3.60 log10 for AHP (P < 0.0001), 1.66 log10 for HAL (P < 0.0001) and 0.32 log10 for FOG (P = 0.004). QAC, AHP and HAL reduced MRSP cfu by 99.97%, 99.98% and 97.81%, respectively. FOG reduced cfu by 52.14%.
Conclusions And Clinical Importance:
QAC and AHP effectively disinfected surfaces inoculated with MRSP. Although HAL provided lower MRSP reduction, it may be considered clinically acceptable. FOG as a sole means of MRSP disinfection was not supported yet may have utility as an adjunctive disinfectant in clinical areas with bacterial densities lower than our experimental inoculum.
Insights
Quaternary ammonium (QAC) and accelerated hydrogen peroxide (AHP) effectively disinfect surfaces against meticillin-resistant Staphylococcus pseudintermedius (MRSP). Hydrogen peroxide and silver (HAL) showed moderate efficacy, while fogging (FOG) was less effective as a sole disinfectant.
Area of Science:
- Veterinary medicine
- Infectious disease control
- Environmental hygiene
Background:
- Meticillin-resistant Staphylococcus pseudintermedius (MRSP) poses a significant risk for nosocomial infections.
- Current disinfectants lack specific label claims against MRSP, necessitating efficacy evaluation.
Purpose of the Study:
- To assess the effectiveness of quaternary ammonium (QAC), accelerated hydrogen peroxide (AHP), hydrogen peroxide and silver (HAL), and a hydrogen peroxide and silver fogging system (FOG) against MRSP.
Main Methods:
- MRSP-inoculated surfaces were treated with QAC, AHP, or HAL under recommended contact times.
- Fogging efficacy was evaluated by placing inoculated samples in a sealed room during the FOG treatment.
- Bacterial reduction was quantified by comparing post-treatment counts to controls.
Main Results:
- QAC and AHP achieved significant MRSP reduction (over 99.97%).
- HAL demonstrated a 97.81% reduction, potentially clinically acceptable.
- The FOG system showed limited efficacy (52.14% reduction) as a standalone disinfection method.
Conclusions:
- QAC and AHP are effective disinfectants for surfaces contaminated with MRSP.
- HAL may offer acceptable clinical efficacy, while FOG requires adjunctive use for comprehensive MRSP control.
More Related Videos
11:47Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
14:17The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
Published on: June 29, 2014
Related Concept Videos
01:28Antimicrobial Effectiveness
01:23Hand hygiene
Hand washing...
01:26Physical Methods for Controlling Microbial Growth: Radiation and Filtration
01:27Chemical Agents for Microbial Control
01:30Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:30Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...