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A sensitive homogenous aptasensor based on tetraferrocene labeling for thrombin detection.

Lin Cheng1, Chen Xu1, Hanfeng Cui1

  • 1The Affiliated Hospital, Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, JiangXi, 330004, China.

Analytica Chimica Acta
|April 22, 2020
PubMed
Summary

A new electrochemical aptasensor detects thrombin using tetraferrocene signal amplification. This simple, electrode-free sensor achieves highly sensitive and specific thrombin detection, crucial for clinical diagnostics.

Keywords:
Electrochemical aptasensorHomogeneousModification-freeTetraferroceneThrombin

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Biochemistry

Background:

  • Thrombin is a key biomarker in hemostasis and thrombosis.
  • Accurate and sensitive detection of thrombin is critical for diagnosing and managing coagulation disorders.
  • Existing thrombin detection methods often require complex procedures or lack sufficient sensitivity and specificity.

Purpose of the Study:

  • To develop a novel homogeneous electrochemical aptasensor for sensitive and specific thrombin detection.
  • To utilize tetraferrocene as a signal amplification marker for enhanced detection.
  • To establish an electrode-modification-free sensing strategy for simplified operation.

Main Methods:

  • Construction of a homogeneous electrochemical aptasensor using a stem-loop aptamer probe labeled with tetraferrocene and thiol.
  • Electrically-assisted self-assembly of the aptamer probe onto the electrode surface upon thrombin binding.
  • Detection of thrombin using differential pulse voltammetry (DPV) to measure the electrochemical signal of tetraferrocene.

Main Results:

  • The aptasensor demonstrated a low detection limit of 0.126 pM for thrombin.
  • The sensor exhibited high specificity, distinguishing thrombin from single-mismatch sequences and common interfering proteins.
  • The tetraferrocene label provided significant signal amplification, enabling rapid thrombin detection.

Conclusions:

  • The developed electrochemical aptasensor offers a simple, sensitive, and specific method for thrombin detection.
  • The electrode-modification-free design and tetraferrocene amplification represent a promising advancement in biosensor technology.
  • This approach has potential applications in clinical diagnostics and biomedical research for coagulation studies.