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Updated: Dec 23, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Genomic characterisation of a newly identified badnavirus infecting ivy (Hedera helix)
Rachelle Bester1, Johan T Burger1, Hans J Maree2,3
1Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Abstract:
High-throughput sequencing (HTS) was used to investigate ringspots on ivy (Hedera helix) leaves. De novo assembly of HTS data generated from a total RNA extract from these leaves yielded a contig with sequence similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 8,885 nucleotides and has three open reading frames (ORFs). Genome organisation and phylogenetic analysis identifies this newly identified virus as a new member of the genus Badnavirus for which we propose the name "ivy ringspot-associated virus" (IRSaV).
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