β-Cyclodextrin-/AuNPs-functionalized covalent organic framework-based magnetic sorbent for solid phase extraction and...

Area of Science:

• Materials Science
• Analytical Chemistry
• Environmental Science

Background:

• Sulfonamides are widely used veterinary drugs with potential health risks.
• Accurate detection of sulfonamides in food matrices is crucial for consumer safety.
• Existing methods for sulfonamide extraction can be time-consuming and complex.

Purpose of the Study:

• To develop and validate a novel magnetic solid-phase extraction (MSPE) sorbent for trace sulfonamide determination.
• To optimize the MSPE method for efficient extraction of sulfonamides from meat samples.
• To evaluate the performance of the developed method using High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS).

Main Methods:

• Synthesis of β-cyclodextrin-functionalized magnetic covalent organic framework (Fe3O4@COF@Au-β-CD) nanoparticles.
• Optimization of MSPE parameters including pH, extraction time, and elution solvent.
• Analysis of sulfonamides in meat samples using HPLC-MS/MS.

Main Results:

• The synthesized Fe3O4@COF@Au-β-CD sorbent demonstrated efficient adsorption of sulfonamides.
• Adsorption kinetics followed a pseudo-second-order model, indicating chemisorption.
• The optimized MSPE-HPLC-MS/MS method exhibited excellent linearity (R² ≥ 0.9936) and low limits of detection (0.8–1.6 μg kg⁻¹).
• High recoveries (78.9–112.0%) and low relative standard deviations (<10%) were achieved for sulfonamide quantification in real meat samples.

Conclusions:

• The developed Fe3O4@COF@Au-β-CD based MSPE method is highly effective for trace sulfonamide extraction from meat.
• This approach offers a sensitive, rapid, and reliable tool for sulfonamide monitoring in food safety.
• The method shows significant potential for application in analyzing sulfonamides in diverse food and environmental samples.