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Updated: Dec 23, 2025

Cell Population Analyses During Skin Carcinogenesis
Published on: August 21, 2013
Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin
1Centre for Skin Sciences, School of Chemistry and Biosciences, Faculty of Life Science, University of Bradford, Bradford, UK. a.mardaryev@bradford.ac.uk.
Abstract:
Two-stage chemical carcinogenesis method is widely used to elucidate genetic and molecular changes that lead to skin cancer development, as well as to test chemotherapeutic properties of novel drugs. This protocol allows researchers to reliably induce benign papilloma development and their conversion to squamous cell carcinoma in the skin of susceptible mouse strains in response to a single dose of carcinogen 2,4-dimethoxybenzaldehyde (DMBA) and repetitive applications of tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA).
![Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60445.jpg&w=3840&q=50)
