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Updated: Dec 23, 2025
RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
Published on: September 27, 2016
[Biology behavior of head and neck squamous cell cancer cells changes after knocking down heat shock protein 27]
Zhen-Kun Zhu1, Yu-Shang Wang1, Xin Xu1
1Dept. of Implantology, School and Hospital of Stomatology, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.
Objective:
This study aimed to observe the metastatic behavior of head and neck squamous cell carcinoma cells after knocking down heat shock protein (Hsp) 27.
Methods:
The experiment was divided into three groups: the lentivirus vector plasmid of pLenti-shRNA-Hsp27 was transfected into UM-SCC-22B cells as experimental group (shHsp27 group), routine culture of UM-SCC-22B cells as blank control (ctrl group), UM-SCC-22B cells transfection of pLenti-shRNA-ctrl lentivirus vector as negative control (shctrl group). Through real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot assay to detect the mRNA expression of Hsp27 in three groups. MTS assay was performed to detect cell-proliferation changes, wounding healing assay was performed to detect cell-migration changes, and Matrigel Transwell invasion assay was performed to detect cell-invasion changes.
Results:
The expression of Hsp27 in shHsp27 group decreased signifi-cantly; MTS assay showed that UM-SCC-22B before and after Hsp27 knockdown had similar proliferation rates after being cultured for 24 or 48 h. Compared with the ctrl group, the shHsp27 group decreased the metastatic behavior by 4.38-fold in migration and 2.03-fold in cell invasion.
Conclusions:
Stably transfected lentivirus vector plasmid of pLenti-shRNA-Hsp27 can efficiently decrease Hsp27 expression and reduce the metastasis ability of UM-SCC-22B.
Insights
Knocking down heat shock protein (Hsp) 27 in head and neck squamous cell carcinoma cells significantly reduced their metastatic behavior. This finding suggests Hsp27 is a potential therapeutic target for reducing cancer spread.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Head and neck squamous cell carcinoma (HNSCC) is a prevalent cancer.
- Heat shock protein 27 (Hsp27) is implicated in cancer progression and metastasis.
Purpose of the Study:
- To investigate the role of Hsp27 in the metastatic behavior of HNSCC cells.
- To determine if Hsp27 knockdown affects HNSCC cell migration and invasion.
Main Methods:
- UM-SCC-22B HNSCC cells were transfected with shRNA targeting Hsp27.
- Quantitative PCR and Western blot assessed Hsp27 expression.
- Cell proliferation, migration (wound healing), and invasion (Matrigel Transwell) assays were performed.
Main Results:
- Hsp27 expression was significantly reduced in the shHsp27 group.
- Cell proliferation rates remained similar across groups.
- Hsp27 knockdown decreased cell migration by 4.38-fold and invasion by 2.03-fold.
Conclusions:
- Stable knockdown of Hsp27 effectively reduces the metastatic potential of HNSCC cells.
- Hsp27 inhibition represents a promising strategy for controlling HNSCC metastasis.
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