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Selective PP2A Enhancement through Biased Heterotrimer Stabilization

Daniel Leonard1, Wei Huang2, Sudeh Izadmehr3

  • 1Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA; Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

Cell
|April 22, 2020
PubMed

Insights

A novel molecule, DT-061, stabilizes the B56α-PP2A enzyme, enhancing its activity against cancer targets like c-Myc. This discovery offers new therapeutic strategies for diseases involving protein phosphatase 2A (PP2A) dysfunction.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Protein phosphatase 2A (PP2A) dysfunction is implicated in various diseases, including cancer.
  • PP2A's activity is modulated by over 40 regulatory B subunits, influencing its substrate specificity and assembly into heterogeneous heterotrimers.
  • Understanding PP2A complex assembly and regulation is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the mechanism of action of the small molecule DT-061.
  • To elucidate how DT-061 stabilizes the B56α-PP2A holoenzyme.
  • To provide insights into PP2A complex assembly and identify therapeutic targets.

Main Methods:

  • 3.6 Å structural analysis of the DT-061 bound B56α-PP2A holoenzyme.
  • Investigation of molecular interactions between DT-061 and PP2A subunits.
  • Assessment of DT-061's effect on PP2A activity and substrate dephosphorylation.

Main Results:

  • DT-061 specifically stabilizes the B56α-PP2A holoenzyme in an active conformation.
  • The molecule engages all three PP2A subunits, preventing holoenzyme dissociation.
  • DT-061 facilitates the dephosphorylation of specific substrates, including the oncogenic protein c-Myc.

Conclusions:

  • DT-061 employs a unique interfacial stabilization mechanism for therapeutic targeting.
  • The findings offer fundamental insights into PP2A complex assembly and regulation.
  • This study aids in developing novel phosphatase-based therapeutics for diseases driven by aberrant protein phosphorylation.
Keywords:
B56αPP2ASMAPc-Myccancercryo-EMphosphatasesmall molecule

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